Methanobrevibacter ruminantium as an indicator of domesticated ruminant fecal pollution in surface waters

["Shannon Briggs" <briggssl@michigan.gov>]

please see abstract at
http://aem.asm.org/cgi/content/abstract/AEM.00911-07v1?ct=ct
 
Methanobrevibacter ruminantium as an indicator of domesticated ruminant
fecal pollution in surface waters 

Jennifer A. Ufnar*, Shiao Y. Wang, David F. Ufnar, and R. D. Ellender 
Department of Biological Sciences, The University of Southern
Mississippi, Hattiesburg, MS 39406, Department of Geography and Geology,
The University of Southern Mississippi, Hattiesburg, MS 39406

* To whom correspondence should be addressed. Email:
jennifer.ufnar@usm.edu. 

   Abstract 

A PCR-based assay (Mrnif) targeting the nifH gene of Methanobrevibacter
ruminantium was developed to detect fecal pollution from domesticated
ruminants in environmental water samples. The assay produced the
expected amplification product only when the reaction contained DNA
extracted from M. ruminantium pure culture, bovine (80%), sheep (100%)
and goat feces (75%), and water samples from a bovine waste lagoon
(100%) and a creek contaminated with bovine lagoon waste (100%). The
assay appears to be specific and sensitive and can distinguish between
domesticated and non-domesticated ruminant fecal pollution in
environmental samples.


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