[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]

RE: DNA testing, or other analysis



I have been involved in attempting to address the beach closing issue for several years and there are various methods for microbial source tracking (MST) with varying costs depending on accuracy and speed desired. One of the better sources for the services is the USGS Research Laboratory in the Dunes National Lakeshore. They have been studying beach closure issues for years including doing MST regarding bacteria at beaches and predictive modeling. Greg Olyphant of Indiana University ahs also been involved in predictive model development for beaches in suthern Lake Michigan. The significant sources for e-coli at a beach in southern Lake Michigan, in varying amounts depending on each beach, include tributaries receiving non-point source run-off (Trail Creek); e-coli which reproduces itself in beach sand; e-coli in the sand from birds, dogs, etc.; e-coli in algae in the near shore water; and swimmers. As far as the contibution from the tributaries, studies regarding stream similar to Trail Creek have shown that most of the bacteria in the tributaries is of non-human origin. Unfortunately, there is a health risk from non-human sources (Wendy's hamburgers, Cryptosporidium outbreaks in Milwaukee and Walkerton, Canada) which amy be even greater than from human sources.  
 
Gary did MST studies at one of its beaches and the Little Calumet River, which receives a lot of CSO discharge, and the percentage of e-coli of human origin at the beach was less than 10% and in the river the approximate percentages were 10% dry weather, 20% wet weather. The remainder was various wild and domesticated animals.
 
James B. Meyer
MEYER & WYATT, P.C.
363 South Lake Street
Gary, IN  46403
Telephone: (219) 938-0800
Facsimile: (219) 939-3070
E-Mail Address:  jmeyer@meyerwyattpc.com

CONFIDENTIALITY NOTICE:  This e-mail and any attachments are for the exclusive and confidential use of the intended recipient, please do not read, distribute or take any action in reliance upon this message.  If you have received this in error, please notify us immediately by return e-mail and promptly delete this message and its attachments from your computer system. We do not waive attorney-client or work product privilege by the transmission of this message.



-----Original Message-----
From: owner-beachnet@great-lakes.net [mailto:owner-beachnet@great-lakes.net]On Behalf Of Dan Olson
Sent: Thursday, August 16, 2007 8:34 AM
To: beachnet@great-lakes.net
Cc: 'Al Walus'; 'Darrell Garbacik'; 'Chuck Oberlie'
Subject: DNA testing, or other analysis

Good Morning,

 

In the NRDC’s “Testing the Waters” 2007 Report, Washington Park Beach was reported as one of the worst in Indiana for beach closings on Lake Michigan in 2006. We are concerned about this issue and desire a proactive approach to control sources other that tributaries and other long-range problems.

 

For that reason, we ask for your membership’s assistance and advice in determining whether the source of E. coli is human or other mammalian in nature. I seem to recall that there are DNA tests available for distinguishing broad categories. However, there may be better tests to assist us.

 

We are interested in the correct description for the analysis, what laboratories perform this analysis, and approximate price range per sample.

 

Thank you,

 

Dan Olson, Plant Superintendent

Sanitary District of Michigan City