Rapid QPCR Methods

[Rachel Noble <rtnoble@email.unc.edu>]

Dear all,

As most of you know my lab has been working for some time to develop 
rapid QPCR based methods for recreational (and drinking water).  We have 
two methods, one for E. coli and one for Enterococcus that we have 
developed for recreational waters, in conjunction with Cepheid, Inc, and 
they are sold in a dry bead format for QPCR.  Those methods were 
originally developed and optimized on the Smart Cycler II (QPCR machine 
from Cepheid), but there are cheaper, higher throughput machines out 
there that we have been testing the assays on to make sure that they 
still work comparably.  We are currently compiling our comparison 
information, and are wondering, have any of you conducted these QPCR 
analyses with machines other than the Smart Cycler II, and have you done 
so by using side by side "traditional" methods of testing (like IDEXX 
kits or something). If you have and you would be willing to share your 
data with us, we will compile our information and share it all to the 
larger BEACH community.  If folks are interested in conducting such 
comparisons, but have not to date, we are willing to share all of our 
protocols, etc. for sample processing.

Best
Rachel Noble

--
Rachel T. Noble, Ph. D.
Associate Professor
University of North Carolina at Chapel Hill
Institute of Marine Sciences
3431 Arendell St.
Morehead City, NC 28557 USA
rtnoble@email.unc.edu
+1 252.726.6841 x150 (tele)
+1 252.726.2426 (fax)


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